• May 30, 2021
hematoonkologia

Izolacja ludzkiego genu, który hamuje

Izolacja ludzkiego genu, który hamuje zakażenie HIV-1 i jest tłumiony przez wirusowe białko Vif. 

Viruses have developed numerous non-immune methods to counteract host-mediated mechanisms that confer resistance to an infection. The Vif (virion infectivity issue) proteins are encoded by primate immunodeficiency viruses, most notably human immunodeficiency virus-1 (HIV-1). These proteins are potent regulators of virus an infection and replication and are consequently important for pathogenic infections in vivo. HIV-1 Vif appears to be required in the course of the late levels of virus manufacturing for the suppression of an innate antiviral phenotype that resides in human T lymphocytes.

Thus, within the absence of Vif, expression of this phenotype renders progeny virions non-infectious. Right here, we describe a singular mobile gene, CEM15, whose transient or secure expression in cells that don’t usually specific CEM15 recreates this phenotype, however whose antiviral motion is overcome by the presence of Vif. As a result of the Vif:CEM15 regulatory circuit is important for HIV-1 replication, perturbing the circuit could also be a promising goal for future HIV/AIDS therapies.

hematoonkologia
hematoonkologia

Vpreb3 3'UTR GFP Stable Cell Line

TU172114 1.0 ml Ask for price

VPREB3 3'UTR GFP Stable Cell Line

TU078275 1.0 ml
EUR 1521

Vpreb3 3'UTR Luciferase Stable Cell Line

TU122114 1.0 ml Ask for price

VPREB3 3'UTR Luciferase Stable Cell Line

TU028275 1.0 ml
EUR 1521

HEK-293T cells

T0011002 One Frozen vial
EUR 455

TARGATT? Knock-in CHO Generation Kit (Master Cell Line)

AST-1200 1 Kit Ask for price
Description: 12 month

TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line)

AST-1300 1 Kit Ask for price
Description: 12 month

HEK-293T Telomerase Over-Expressing Cell Pellet

abx069991-1Pellet 1 Pellet
EUR 398

Basic HR Targeting Vector [MCS1-LoxP-MCS2-MCS3-pA-LoxP-MCS4] for Gene Knock-In/Out

HR100PA-1 10 ug
EUR 938

VPREB3 siRNA

20-abx939489
  • EUR 551.00
  • EUR 732.00
  • 15 nmol
  • 30 nmol

VPREB3 Antibody

1-CSB-PA025888GA01HU
  • EUR 597.00
  • EUR 333.00
  • 150ul
  • 50ul
Description: A polyclonal antibody against VPREB3. Recognizes VPREB3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA, WB

VPREB3 Antibody

1-CSB-PA891724LA01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against VPREB3. Recognizes VPREB3 from Human. This antibody is Unconjugated. Tested in the following application: ELISA

VPREB3 Antibody

43594-100ul 100ul
EUR 252

anti-VPREB3

YF-PA26081 50 ul
EUR 334
Description: Mouse polyclonal to VPREB3

anti-VPREB3

YF-PA18600 50 ul
EUR 363
Description: Mouse polyclonal to VPREB3

anti-VPREB3

YF-PA18601 50 ug
EUR 363
Description: Mouse polyclonal to VPREB3

VPREB3 antibody

70R-21261 50 ul
EUR 435
Description: Rabbit polyclonal VPREB3 antibody

VPREB3 Peptide

42-296P 0.1 mg
EUR 338
Description: (IN) VPREB3 Peptide

VPREB3 sgRNA CRISPR Lentivector set (Human)

K2617001 3 x 1.0 ug
EUR 339

293LTV Cell Line

LTV-100 1 vial
EUR 508
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293AD Cell Line

AD-100 1 vial
EUR 461
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 508
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 508
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

Human VPREB3 shRNA Plasmid

20-abx959234
  • EUR 801.00
  • EUR 1121.00
  • 150 µg
  • 300 µg

VPREB3 ELISA KIT|Human

EF004206 96 Tests
EUR 689

VPREB3 Recombinant Protein (Human)

RP034357 100 ug Ask for price

Microplate Swing-Out Centrifuge

abx725026-1Unit 1 Unit
EUR 1476

Clinical Swing-Out Centrifuge

abx725027-1Unit 1 Unit
EUR 1476

Gene Knock-Out HR Targeting Vector w/Single Selection Marker (Blasticidin) and Negative Selection (TK) Against Random Integration

HR720PA-1 10 µg
EUR 1145

293T Transfection Kit (1 mL)

P902 - Ask for price

293T Transfection Kit (0.2 mL)

P902S - Ask for price

293T Transfection Kit (1 mL)

P903 - Ask for price

293T Transfection Kit (0.2 mL)

P903S - Ask for price

293/GFP Cell Line

AKR-200 1 vial
EUR 572
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

T47D/GFP Cell Line

AKR-208 1 vial
EUR 572
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 572
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 572
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 572
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 572

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 572

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 572

VPREB3 Polyclonal Antibody

A61726 100 µg
EUR 570.55
Description: fast delivery possible

Anti-VPREB3 antibody

STJ73463 100 µg
EUR 260

VPREB3 cloning plasmid

CSB-CL891724HU-10ug 10ug
EUR 233
Description: A cloning plasmid for the VPREB3 gene.

anti- VPREB3 antibody

FNab09425 100µg
EUR 585
Description: Antibody raised against VPREB3

VPREB3 Conjugated Antibody

C43594 100ul
EUR 397

Anti-VPREB3 (4H8)

YF-MA18256 100 ug
EUR 363
Description: Mouse monoclonal to VPREB3

Anti-VPREB3 antibody

PAab09425 100 ug
EUR 412

VPREB3 sgRNA CRISPR Lentivector (Human) (Target 1)

K2617002 1.0 ug DNA
EUR 154

VPREB3 sgRNA CRISPR Lentivector (Human) (Target 2)

K2617003 1.0 ug DNA
EUR 154

VPREB3 sgRNA CRISPR Lentivector (Human) (Target 3)

K2617004 1.0 ug DNA
EUR 154

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (GFP+Puro) and Negative Selection (TK) Against Random Integration

HR700PA-1 10 µg
EUR 1145

Gene Knock-Out HR Targeting Vector w/Dual Selection Markers (RFP+Hygro) and Negative Selection (TK) Against Random Integration

HR710PA-1 10 µg
EUR 1145

VPREB3 ORF Vector (Human) (pORF)

ORF011453 1.0 ug DNA
EUR 95

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 572
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 572
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 572
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Gene Knock-Out HR Targeting Vector with TK selection [MCS1-LoxP-EF1?-GFP-T2A-Puro-P2A-hsvTK-pA-LoxP-MCS2]

HR210PA-1 10 ug
EUR 1145

Vpreb3 sgRNA CRISPR Lentivector set (Mouse)

K3123201 3 x 1.0 ug
EUR 339

Vpreb3 sgRNA CRISPR Lentivector set (Rat)

K6154001 3 x 1.0 ug
EUR 339

VPREB3 Antibody, HRP conjugated

1-CSB-PA891724LB01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against VPREB3. Recognizes VPREB3 from Human. This antibody is HRP conjugated. Tested in the following application: ELISA

VPREB3 Antibody, FITC conjugated

1-CSB-PA891724LC01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against VPREB3. Recognizes VPREB3 from Human. This antibody is FITC conjugated. Tested in the following application: ELISA

VPREB3 Antibody, Biotin conjugated

1-CSB-PA891724LD01HU
  • EUR 317.00
  • EUR 335.00
  • 100ug
  • 50ug
Description: A polyclonal antibody against VPREB3. Recognizes VPREB3 from Human. This antibody is Biotin conjugated. Tested in the following application: ELISA

VPREB3 Recombinant Protein (Rat)

RP236981 100 ug Ask for price

VPREB3 Recombinant Protein (Mouse)

RP184868 100 ug Ask for price

TARGATT? Knock-in iPSC Genotyping Kit

AST-1102 1 Kit Ask for price
Description: 12 month

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 920
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

PinPoint-FC 293T Platform Cell Line for Targeted Gene Insertion (with PinPoint site already placed)

PIN320A-1 >2x10^5 cells
EUR 3104

Vpreb3 sgRNA CRISPR Lentivector (Mouse) (Target 1)

K3123202 1.0 ug DNA
EUR 154

Vpreb3 sgRNA CRISPR Lentivector (Mouse) (Target 2)

K3123203 1.0 ug DNA
EUR 154

Vpreb3 sgRNA CRISPR Lentivector (Mouse) (Target 3)

K3123204 1.0 ug DNA
EUR 154

Vpreb3 sgRNA CRISPR Lentivector (Rat) (Target 1)

K6154002 1.0 ug DNA
EUR 154

Vpreb3 sgRNA CRISPR Lentivector (Rat) (Target 2)

K6154003 1.0 ug DNA
EUR 154

Vpreb3 sgRNA CRISPR Lentivector (Rat) (Target 3)

K6154004 1.0 ug DNA
EUR 154

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 345
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

VPREB3 Polyclonal Antibody, Biotin Conjugated

A61727 100 µg
EUR 570.55
Description: reagents widely cited

VPREB3 Polyclonal Antibody, FITC Conjugated

A61728 100 µg
EUR 570.55
Description: Ask the seller for details

VPREB3 Polyclonal Antibody, HRP Conjugated

A61729 100 µg
EUR 570.55
Description: The best epigenetics products

Polyclonal VPREB3 Antibody (internal region)

APG00623G 0.1mg
EUR 484
Description: A polyclonal antibody raised in Goat that recognizes and binds to Human VPREB3 (internal region). This antibody is tested and proven to work in the following applications:

Vpreb3 ORF Vector (Mouse) (pORF)

ORF061624 1.0 ug DNA
EUR 506

Vpreb3 ORF Vector (Rat) (pORF)

ORF078995 1.0 ug DNA
EUR 506

TARGATT? Knock-in iPSC Quick Knockin Kit

AST-1101 1 Kit Ask for price
Description: 12 month

VPREB3 Protein Vector (Human) (pPB-C-His)

PV045809 500 ng
EUR 329

VPREB3 Protein Vector (Human) (pPB-N-His)

PV045810 500 ng
EUR 329

VPREB3 Protein Vector (Human) (pPM-C-HA)

PV045811 500 ng
EUR 329

VPREB3 Protein Vector (Human) (pPM-C-His)

PV045812 500 ng
EUR 329

Out At First Protein Homolog (OAF) Antibody

abx027798-400ul 400 ul
EUR 523

Out At First Protein Homolog (OAF) Antibody

abx027798-80l 80 µl
EUR 286

Szybka izolacja antygenów z komórek z adsorbentem gronkowcowym białko A-przeciwciało: parametry interakcji kompleksów przeciwciało-antygen z białkiem A. 

The Cowan I pressure of the bacterium Staphylococcus aureus has been used as an adsorbent for antibodies complexed with radiolabeled antigens from cell lysates. This utility is superior as a superior different to different strategies of immune precipitation for the isolation of antigens. It exploits the excessive adsorption capability for IgG molecules by protein A molecules on the cell partitions of sure strains of staphylococci, together with the advantageous sedimentation properties of the micro organism. The interplay of immune complexes with the adsorbent was outlined initially utilizing a mannequin system of bovine serum albumin with a excessive extra of rabbit anti-bovine serum albumin antibodies (IgG).

The uptake of immune complexes below these situations was extraordinarily speedy, occurring inside seconds, whereas most binding of free IgG was a lot slower. As well as, as soon as sure the complexed antigen couldn’t be displaced from the adsorbent both by massive quantities of regular IgG or by further free antibody. Antigen might be eluted virtually utterly from the inert adsorbent for analytic or preparative functions with a wide range of solvent methods, such because the detergent SDS in mixture with urea and excessive temperature, and impartial salts with robust lyotropic salting in properties.

The efficacy of the protein A-antibody adsorption method was examined in direct comparisons with a traditional double antibody precipitation technique for the isolation of mouse lymphocyte IgM. The bacterial adsorbent not solely had a definite benefit in velocity of antigen isolation, however analyses by polyacrylamide gel electrophoresis in SDS additionally revealed constantly larger antigen recoveries, decrease ranges of background radioactivity, and an absence of different cell elements which can nonspecifically bind to and complicate analyses utilizing standard immune precipitates.

Antygen CD4 (T4) jest podstawowym składnikiem receptora retrowirusa AIDS. 

Acquired immune deficiency syndrome (AIDS) is characterised by opportunistic infections and by ‘opportunistic neoplasms’ (for instance, Kaposi’s sarcoma). Persistent generalized lymphadenopathy (PGL) is epidemiologically related to AIDS, particularly in male homosexuals. A subset of T lymphocytes optimistic for the CD4 antigen (additionally termed T4 antigen), is depleted in AIDS and PGL sufferers. A retrovirus present in T-cell cultures from these sufferers is strongly implicated within the aetiology of AIDS due to the excessive frequency of isolation and the prevalence of particular antibodies within the sufferers. Right here we’ve detected cell-surface receptors for the AIDS retrovirus (human T-cell leukaemia virus-III (HTLV-III) and lymphadenopathy-associated virus-1 (LAV-1) isolates) by testing the susceptibility of cells to an infection with pseudotypes of vesicular stomatitis virus bearing retroviral envelope antigens, and by the formation of multinucleated syncytia on mixing virus-producing cells with receptor-bearing cells.

Receptors had been current solely on cells expressing CD4 antigen; amongst 155 monoclonal antibodies examined, every of the 14 anti-CD4 antibodies inhibited formation of syncytia and blocked pseudotypes. Productive an infection of CD4+ cells with HTLV-III or LAV-1 markedly diminished cell-surface expression of CD4. In distinction, receptors for HTLV-I and HTLV-II weren’t restricted to CD4+ cells, weren’t blocked by anti-CD4 antibodies; cells productively contaminated with HTLV-I and HTLV-II expressed floor CD4. Therefore, we conclude that the CD4 antigen is a necessary and particular part of the receptor for the causative agent of AIDS.

Komórkowe i molekularne mechanizmy zwłóknienia. 

Fibrosis is outlined by the overgrowth, hardening, and/or scarring of assorted tissues and is attributed to extra deposition of furthercellular matrix elements together with collagen. Fibrosis is the top results of continual inflammatory reactions induced by a wide range of stimuli together with persistent infections, autoimmune reactions, allergic responses, chemical insults, radiation, and tissue damage. Though present therapies for fibrotic illnesses reminiscent of idiopathic pulmonary fibrosis, liver cirrhosis, systemic sclerosis, progressive kidney illness, and cardiovascular fibrosis usually goal the inflammatory response, there’s accumulating proof that the mechanisms driving fibrogenesis are distinct from these regulating irritation. In truth, some research have recommended that ongoing irritation is required to reverse established and progressive fibrosis.

The important thing cellular mediator of fibrosis is the myofibroblast, which when activated serves as the first collagen-producing cell. Myofibroblasts are generated from a wide range of sources together with resident mesenchymal cells, epithelial and endothelial cells in processes termed epithelial/endothelial-mesenchymal (EMT/EndMT) transition, in addition to from circulating fibroblast-like cells known as fibrocytes which can be derived from bone-marrow stem cells. Myofibroblasts are activated by a wide range of mechanisms, together with paracrine indicators derived from lymphocytes and macrophages, autocrine components secreted by myofibroblasts, and pathogen-associated molecular patterns (PAMPS) produced by pathogenic organisms that work together with sample recognition receptors (i.e. TLRs) on fibroblasts.

Cytokines (IL-13, IL-21, TGF-beta1), chemokines (MCP-1, MIP-1beta), angiogenic components (VEGF), progress components (PDGF), peroxisome proliferator-activated receptors (PPARs), acute part proteins (SAP), caspases, and elements of the renin-angiotensin-aldosterone system (ANG II) have been recognized as necessary regulators of fibrosis and are being investigated as potential targets of antifibrotic medication. This evaluate explores our present understanding of the cellular and molecular mechanisms of fibrogenesis.

Predykcyjne korelaty odpowiedzi na przeciwciało anty-PD-L1 MPDL3280A u pacjentów z rakiem.

 The event of human most cancers is a multistep course of characterised by the buildup of genetic and epigenetic alterations that drive or replicate tumour development. These adjustments distinguish most cancers cells from their regular counterparts, permitting tumours to be acknowledged as overseas by the immune system. Nonetheless, tumours are not often rejected spontaneously, reflecting their capability to keep up an immunosuppressive microenvironment. Programmed death-ligand 1 (PD-L1; additionally known as B7-H1 or CD274), which is expressed on many most cancers and immune cells, performs an necessary half in blocking the ‘most cancers immunity cycle’ by binding programmed death-1 (PD-1) and B7.1 (CD80), each of that are unfavorable regulators of T-lymphocyte activation. Binding of PD-L1 to its receptors suppresses T-cell migration, proliferation and secretion of cytotoxic mediators, and restricts tumour cell killing.

The PD-L1-PD-1 axis protects the host from overactive T-effector cells not solely in most cancers but in addition throughout microbial infections. Blocking PD-L1 ought to due to this fact improve anticancer immunity, however little is understood about predictive components of efficacy. This research was designed to judge the security, exercise and biomarkers of PD-L1 inhibition utilizing the engineered humanized antibody MPDL3280A. Right here we present that throughout a number of most cancers varieties, responses (as evaluated by Response Analysis Standards in Stable Tumours, model 1.1) had been noticed in sufferers with tumours expressing excessive ranges of PD-L1, particularly when PD-L1 was expressed by tumour-infiltrating immune cells. Moreover, responses had been related to T-helper kind 1 (TH1) gene expression, CTLA4 expression and the absence of fractalkine (CX3CL1) in baseline tumour specimens. Collectively, these information recommend that MPDL3280A is best in sufferers during which pre-existing immunity is suppressed by PD-L1, and is re-invigorated on antibody therapy.

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