• 25 maja, 2021
hematoonkologia

Izolacja retrowirusa limfotropowego T od pacjenta

Izolacja retrowirusa limfotropowego T od pacjenta z ryzykiem zespołu nabytego niedoboru odporności (AIDS). 

A retrovirus belonging to the household of just lately found human T-cell leukemia viruses (HTLV), however clearly distinct from every earlier isolate, has been remoted from a Caucasian affected person with indicators and signs that usually precede the acquired immune deficiency syndrome (AIDS). This virus is a typical type-C RNA tumor virus, buds from the cell membrane, prefers magnesium for reverse transcriptase exercise, and has an inside antigen (p25) just like HTLV p24.

Antibodies from serum of this affected person react with proteins from viruses of the HTLV-I subgroup, however type-specific antisera to HTLV-I don’t precipitate proteins of the brand new isolate. The virus from this affected person has been transmitted into wire blood lymphocytes, and the virus produced by these cells is just like the unique isolate. From these research it’s concluded that this virus in addition to the earlier HTLV isolates belong to a normal household of T-lymphotropic retroviruses which are horizontally transmitted in people and could also be concerned in a number of pathological syndromes, together with AIDS.

hematoonkologia
hematoonkologia

CXCL1 CRISPR Knock Out L3.6pl Cell Line

T6221 1x10^6 cells / 1.0 ml Ask for price

Ccl2 CRISPR Knock Out CT26 Cell Line

T9611 1x10^6 cells / 1.0 ml Ask for price

TRPV6 CRISPR Knock Out Human PC3-M Cell Line

T6219 1x10^6 cells / 1.0 ml Ask for price

FOLR CRISPR Knock Out Human OVCAR3-A1 Cell Line

T6229 1x10^6 cells / 1.0 ml Ask for price

MUC16 CRISPR Knock Out Human OVCAR3-A1 Cell Line

T6230 1x10^6 cells / 1.0 ml Ask for price

NR2F6 CRISPR Knock Out Human Jurkat E6.1 Cell Line - Clone T3-10

T6822 1x10^6 cells / 1.0 ml Ask for price

Aldh2 CRISPR Knock Out L929 Cell Line - Clone T3-20

T9692 1x10^6 cells / 1.0 ml Ask for price

TREM2 CRISPR Knock Out Human THP-1 Cell Line - Clone T1-9

T6810 1x10^6 cells / 1.0 ml Ask for price

PTPN6 CRISPR Knock Out Human THP-1 Cell Line - Clone T3-1

T6813 1x10^6 cells / 1.0 ml Ask for price

PTPN11 CRISPR Knock Out NIH/3T3 Cell Line - Clone T4-9

T6803 1x10^6 cells / 1.0 ml Ask for price

METTL3 CRISPR Knock Out Caki-1 Cell Line - Clone T2-16

T9623 1x10^6 cells / 1.0 ml
EUR 3950

Nacc1 CRISPR Knock Out ID8 Cell Line (Mouse) - Clone T1-23

T9627 1x10^6 cells / 1.0 ml Ask for price

osbpl2 CRISPR Knock Out NIH/3T3 Cell Line - T1 Polyclonal Pool

T9682 1x10^6 cells / 1.0 ml Ask for price

GLT1 CRISPR Knock Out Rat AST Cell Line - Clone T2-1

T9678 1x10^6 cells / 1.0 ml Ask for price

PRSS21 CRISPR Knock Out Human OVCAR8 Cell Line - Clone T2 C8

T6811 1x10^6 cells / 1.0 ml Ask for price

SEC61G CRISPR Knock Out HCC-LM3 Cell Line - Clone T2-17-5

T9746 1x10^6 cells / 1.0 ml Ask for price

Aifm1 CRISPR Knock Out Mouse L929 Cell Line - Clone T2-15-3

T9674 1x10^6 cells / 1.0 ml Ask for price

MSLN CRISPR Knock Out Human OVCAR3-A1 Cell Line - Clone T2 C4

T6820 1x10^6 cells / 1.0 ml Ask for price

CARMIL1 CRISPR Knock Out Immortalized Human Gingival Fibroblast - hTERT Cell Line - T1-2

T6825 1x10^6 cells / 1.0 ml Ask for price

hPIM1 CRISPR Knock Out SACC-83 Cell Line-T3-9

T9787 1x10^6 cells / 1.0 ml Ask for price

Dcx CRISPR Knock Out C6 Cell Line (Rat) - Clone sg4-1

T9624 1x10^6 cells / 1.0 ml
EUR 3950

Cell line gene knock-in and knock-out

S301 -
EUR 15000

Human LY96 knockout cell line

ABC-KH8810 1 vial Ask for price
Description: Human LY96 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY96 knockdown cell line

ABC-KD8810 1 vial Ask for price
Description: Human LY96 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

SIRT1 Knockout Stable 293T Cell Line

T3176 1x10^6 cells / 1.0 ml
EUR 3950

Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T1-19

T9511 1x10^6 cells / 1.0 ml
EUR 3950

Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T3-3

T9529 1x10^6 cells/1.0ml
EUR 3950

T47D/GFP Cell Line

AKR-208 1 vial
EUR 686.4
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line

T3187 1x10^6 cells / 1.0 ml
EUR 3950

293T [HEK-293T] Cell Line

CL-0005 1×10⁶ cells/vial
EUR 420
Description: Homo sapiens, Human

293T Cell Line

LV010 1x10^6
EUR 450

293T/17 Cell Line

CL-0469 1×10⁶ cells/vial
EUR 500
Description: Homo sapiens, Human

293T Cell Lysate (Human embryonic kidney cell line)

LF-R0006 200 ul
EUR 103.2
Description: 293T (Human embryonic kidney cell line) Whole Cell Lysate

293AD Cell Line

AD-100 1 vial
EUR 553.2
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area.

293AAV Cell Line

AAV-100 1 vial
EUR 609.6
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area.

293LTV Cell Line

LTV-100 1 vial
EUR 609.6
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area.

293RTV Cell Line

RV-100 1 vial
EUR 609.6
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area.

Cas9 Expressing 293T Cell Line

T3251 1x10^6 cells / 1.0 ml
EUR 795

293/GFP Cell Line

AKR-200 1 vial
EUR 686.4
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

A549/GFP Cell Line

AKR-209 1 vial
EUR 686.4
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

HeLa/GFP Cell Line

AKR-213 1 vial
EUR 686.4
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

293/Cas9 Cell Line

AKR-5110 1 vial
EUR 686.4

HeLa/Cas9 Cell Line

AKR-5111 1 vial
EUR 686.4

NIH3T3/GFP Cell Line

AKR-214 1 vial
EUR 686.4
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line.

NIH3T3/Cas9 Cell Line

AKR-5104 1 vial
EUR 686.4

Human LY75 knockout cell line

ABC-KH8806 1 vial Ask for price
Description: Human LY75 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY86 knockout cell line

ABC-KH8808 1 vial Ask for price
Description: Human LY86 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Blank Promoter Stable Expressing Human 293T Cell Line

T9618 1x10^6 cells / 1.0 ml
EUR 3950

MCF-7/Luc Cell Line

AKR-234 1 vial
EUR 686.4
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

SKOV-3/Luc Cell Line

AKR-232 1 vial
EUR 686.4
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line.

Stable 293T-ATD cell line

T6340 1x10^6 cells / 1.0 ml
EUR 3950

OVCAR-5/RFP Cell Line

AKR-254 1 vial
EUR 686.4
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line.

Human LY75 knockdown cell line

ABC-KD8806 1 vial Ask for price
Description: Human LY75 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY86 knockdown cell line

ABC-KD8808 1 vial Ask for price
Description: Human LY86 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY75-CD302 knockout cell line

ABC-KH8807 1 vial Ask for price
Description: Human LY75-CD302 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

PTPN6 Stable Knocked Out THP-1 Cell Line

T6216 1x10^6 cells / 1.0 ml
EUR 3950

SIRT1 Wildtype Stable 293T Cell Line

T3175 1x10^6 cells / 1.0 ml
EUR 3950

Mouse NACC1 CRISPR Stable Knockout ID8 Cell Line - Clone T1-23

T6826 1x10^6 cells / 1.0 ml
EUR 3950

Human LY9 knockout cell line

ABC-KH8809 1 vial Ask for price
Description: Human LY9 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6D knockout cell line

ABC-KH8797 1 vial Ask for price
Description: Human LY6D knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6E knockout cell line

ABC-KH8798 1 vial Ask for price
Description: Human LY6E knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6H knockout cell line

ABC-KH8804 1 vial Ask for price
Description: Human LY6H knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6K knockout cell line

ABC-KH8805 1 vial Ask for price
Description: Human LY6K knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G5B knockout cell line

ABC-KH8799 1 vial Ask for price
Description: Human LY6G5B knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G5C knockout cell line

ABC-KH8800 1 vial Ask for price
Description: Human LY6G5C knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G6C knockout cell line

ABC-KH8801 1 vial Ask for price
Description: Human LY6G6C knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G6D knockout cell line

ABC-KH8802 1 vial Ask for price
Description: Human LY6G6D knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY6G6F knockout cell line

ABC-KH8803 1 vial Ask for price
Description: Human LY6G6F knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human LY75-CD302 knockdown cell line

ABC-KD8807 1 vial Ask for price
Description: Human LY75-CD302 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

PGC-1α/ERRα Stable 293T Cell Line

T3060 1x10^6 cells / 1.0 ml
EUR 385

Platinum-E Retroviral Packaging Cell Line, Ecotropic

RV-101 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Platinum-GP Retroviral Packaging Cell Line, Pantropic

RV-103 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector.

Platinum-A Retroviral Packaging Cell Line, Amphotropic

RV-102 1 vial
EUR 1104
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection.

Total Protein - Murine Embryonic Stem Cell Line D3

CBA-305 500 ?g
EUR 414
Description:
  • Isolated from mouse ES-D3 cell line
  • Presented as 500 µg at 1 mg/mL in NP-40 Solubilization Buffer

Human LY9 knockdown cell line

ABC-KD8809 1 vial Ask for price
Description: Human LY9 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Mouse R-spondin1 Stable 293T Cell Line

T3156 1x10^6 cells / 1.0 ml
EUR 3950

Human LY6D knockdown cell line

ABC-KD8797 1 vial Ask for price
Description: Human LY6D knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6E knockdown cell line

ABC-KD8798 1 vial Ask for price
Description: Human LY6E knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6H knockdown cell line

ABC-KD8804 1 vial Ask for price
Description: Human LY6H knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6K knockdown cell line

ABC-KD8805 1 vial Ask for price
Description: Human LY6K knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G5B knockdown cell line

ABC-KD8799 1 vial Ask for price
Description: Human LY6G5B knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G5C knockdown cell line

ABC-KD8800 1 vial Ask for price
Description: Human LY6G5C knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G6C knockdown cell line

ABC-KD8801 1 vial Ask for price
Description: Human LY6G6C knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G6D knockdown cell line

ABC-KD8802 1 vial Ask for price
Description: Human LY6G6D knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LY6G6F knockdown cell line

ABC-KD8803 1 vial Ask for price
Description: Human LY6G6F knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

PFN2 Stable Expressing 293T Cell Line - Polyclonal

T9606 1x10^6 cells / 1.0 ml
EUR 3950

TARGATT? Knock-in Mouse Cell Line Generation Kit (Master Cell Line)

AST-7001 1 Kit Ask for price
Description: 6 month

BRCA1 CRISPR Stable Knockout Human Fibroblast Cell Line

T6245 1x10^6 cells / 1.0 ml
EUR 3950

Human 293T Cell Lysate Lyophilized

IHU293TTLLY200UG each
EUR 89
Description: Human 293T Cell Lysate Lyophilized

TARGATT? Knock-in iPSC Generation (Master Cell Line)

AST-1100 1 vial of 1X10^6 cells Ask for price
Description: 6 month

YBX1 Stable Knockout Human NK/T Cell Line #T3-16 P6

T9508 1x10^6 cells / 1.0 ml Ask for price

Ly96 3'UTR GFP Stable Cell Line

TU262699 1.0 ml Ask for price

Ly96 3'UTR GFP Stable Cell Line

TU162731 1.0 ml Ask for price

LY96 3'UTR GFP Stable Cell Line

TU062782 1.0 ml
EUR 1672.8

Human CRISP1 knockout cell line

ABC-KH3569 1 vial Ask for price
Description: Human CRISP1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISP2 knockout cell line

ABC-KH3570 1 vial Ask for price
Description: Human CRISP2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISP3 knockout cell line

ABC-KH3571 1 vial Ask for price
Description: Human CRISP3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

PTPRA CRISPR Knockout Immortalized Human Lung Fibroblast Cell Line

T6827 1x10^6 cells / 1.0 ml Ask for price

Human 293T Whole Cell Lysate

LYSATE0032 200ug
EUR 180
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

VEGF Promoter Stable Expressing 293T Cell Line - Clone #2

T9622 1x10^6 cells / 1.0 ml
EUR 3950

Human CRISPLD1 knockout cell line

ABC-KH3572 1 vial Ask for price
Description: Human CRISPLD1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRISPLD2 knockout cell line

ABC-KH3573 1 vial Ask for price
Description: Human CRISPLD2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Ly96 3'UTR Luciferase Stable Cell Line

TU112731 1.0 ml Ask for price

Ly96 3'UTR Luciferase Stable Cell Line

TU212699 1.0 ml Ask for price

LY96 3'UTR Luciferase Stable Cell Line

TU012782 1.0 ml
EUR 1672.8

AAVS1 Positive Control EGIP 293T Reporter Cell Line

CAS606A-1 1 Vial
EUR 726

Acan CRISPR Knockout RCS Cell Line (Rat)

T3000 1x10^6 cells / 1.0 ml Ask for price

TARGATT? Knock-in CHO Generation Kit (Master Cell Line)

AST-1200 1 Kit Ask for price
Description: 12 month

Human CRISP1 knockdown cell line

ABC-KD3569 1 vial Ask for price
Description: Human CRISP1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRISP2 knockdown cell line

ABC-KD3570 1 vial Ask for price
Description: Human CRISP2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRISP3 knockdown cell line

ABC-KD3571 1 vial Ask for price
Description: Human CRISP3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line)

AST-1300 1 Kit Ask for price
Description: 12 month

Human CRISPLD1 knockdown cell line

ABC-KD3572 1 vial Ask for price
Description: Human CRISPLD1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRISPLD2 knockdown cell line

ABC-KD3573 1 vial Ask for price
Description: Human CRISPLD2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

GFAT1 CRISPR Stable Knockout H1299 Cell Line

T6199 1x10^6 cells / 1.0 ml
EUR 3950

EDC4 CRISPR Stable Knockout HEK293T Cell Line

T6241 1x10^6 cells / 1.0 ml
EUR 3950

DJ-1 CRISPR Stable Knockout HeLa Cell Line

T6181 1x10^6 cells / 1.0 ml
EUR 3950

PD-L1 CRISPR Stable Knockout CT26 Cell Line

T6237 1x10^6 cells / 1.0 ml
EUR 3950

PDPN CRISPR Stable Knockout COS-7 Cell Line

T6039 1x10^6 cells / 1.0 ml
EUR 3950

PD-L1 CRISPR Stable Knockout MC38 Cell Line

T6149 1x10^6 cells / 1.0 ml
EUR 3950

LIMD1-/- CRISPR Stable Knockout HeLa Cell Line

T6357 1x10^6 cells / 1.0 ml
EUR 3950

LY96 sgRNA CRISPR Lentivector set (Human)

K1246301 3 x 1.0 ug
EUR 406.8

SLC35A1 CRISPR Stable Knockout CHO-S Cell Line

T6040 1x10^6 cells / 1.0 ml
EUR 3950

CXCL1 & CXCL8 CRISPR Double Knockout L3.6pl Cell Line

T6047 1x10^6 cells / 1.0 ml Ask for price

hCETP shRNA (Target C) Knock Down THP-1 Cell Line-Polyclone

T9789 1x10^6 cells / 1.0 ml Ask for price

AXL CRISPR Stable Knockout HT1080 (1-IV) Cell Line

T6232 1x10^6 cells / 1.0 ml
EUR 3950

AXL CRISPR Stable Knockout HT1080 (3-II) Cell Line

T6233 1x10^6 cells / 1.0 ml
EUR 3950

Human CRIM1 knockout cell line

ABC-KH3563 1 vial Ask for price
Description: Human CRIM1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIP1 knockout cell line

ABC-KH3564 1 vial Ask for price
Description: Human CRIP1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIP2 knockout cell line

ABC-KH3565 1 vial Ask for price
Description: Human CRIP2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIP3 knockout cell line

ABC-KH3566 1 vial Ask for price
Description: Human CRIP3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIPT knockout cell line

ABC-KH3568 1 vial Ask for price
Description: Human CRIPT knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human CRIPAK knockout cell line

ABC-KH3567 1 vial Ask for price
Description: Human CRIPAK knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

LY96 sgRNA CRISPR Lentivector (Human) (Target 1)

K1246302 1.0 ug DNA
EUR 184.8

LY96 sgRNA CRISPR Lentivector (Human) (Target 2)

K1246303 1.0 ug DNA
EUR 184.8

LY96 sgRNA CRISPR Lentivector (Human) (Target 3)

K1246304 1.0 ug DNA
EUR 184.8

Cas9 (CRISPR Associated Protein 9) ELISA Kit

PRB-5079 96 assays
EUR 686.4

Cas13a (CRISPR Associated Protein 13a, C2c2) ELISA Kit

PRB-5091 96 assays
EUR 686.4

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135 96 assays
EUR 985.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric

CBA-135-5 5 x 96 assays
EUR 4027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140 96 assays
EUR 1027.2
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric

CBA-140-5 5 x 96 assays
EUR 4179.6
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325 96 assays
EUR 1027.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible)

CBA-325-5 5 x 96 assays
EUR 4033.2
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis.

Human CRIM1 knockdown cell line

ABC-KD3563 1 vial Ask for price
Description: Human CRIM1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIP1 knockdown cell line

ABC-KD3564 1 vial Ask for price
Description: Human CRIP1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIP2 knockdown cell line

ABC-KD3565 1 vial Ask for price
Description: Human CRIP2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIP3 knockdown cell line

ABC-KD3566 1 vial Ask for price
Description: Human CRIP3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIPT knockdown cell line

ABC-KD3568 1 vial Ask for price
Description: Human CRIPT knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human CRIPAK knockdown cell line

ABC-KD3567 1 vial Ask for price
Description: Human CRIPAK knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest.

Human LIN37 knockout cell line

ABC-KH8476 1 vial Ask for price
Description: Human LIN37 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human KY knockout cell line

ABC-KH8250 1 vial Ask for price
Description: Human KY knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human MB knockout cell line

ABC-KH9065 1 vial Ask for price
Description: Human MB knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human F2 knockout cell line

ABC-KH5009 1 vial Ask for price
Description: Human F2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human F3 knockout cell line

ABC-KH5014 1 vial Ask for price
Description: Human F3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human F5 knockout cell line

ABC-KH5015 1 vial Ask for price
Description: Human F5 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human F7 knockout cell line

ABC-KH5016 1 vial Ask for price
Description: Human F7 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human F8 knockout cell line

ABC-KH5017 1 vial Ask for price
Description: Human F8 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human F9 knockout cell line

ABC-KH5021 1 vial Ask for price
Description: Human F9 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Human GC knockout cell line

ABC-KH5948 1 vial Ask for price
Description: Human GC knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology.

Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych limfocytów pacjenta ze skórnym chłoniakiem T-komórkowym. 

Retrovirus particles with sort C morphology have been present in two T-cell lymphoblastoid cell strains, HUT 102 and CTCL-3, and in recent peripheral blood lymphocytes obtained from a affected person with a cutaneous T-cell lymphoma (mycosis fungoides). The cell strains constantly produce these viruses, that are collectively known as HTLV, pressure CR(HTLV(CR)). Initially, the manufacturing of virus from HUT 102 cells required induction with 5-iodo-2′-deoxyuridine, however the cell line grew to become a constitutive producer of virus at its 56th passage. Cell line CTCL-Three has been a constitutive producer of virus from its second passage in tradition.

Each mature and immature furthercellular virus particles have been seen in thin-section electron micrographs of fastened, pelleted cellular materials; once in a while, typical sort C budding virus particles have been seen. No type of intracellular virus particle has been seen. Mature particles have been 100-110 nm in diameter, consisted of an electron-dense core surrounded by an outer membrane separated by an electron-lucent area, banded at a density of 1.16 g/ml on a steady 25-65% sucrose gradient, and contained 70S RNA and a DNA polymerase exercise typical of viral reverse transcriptase (RT; RNA-dependent DNA nucleotidyltransferase). Underneath sure circumstances of assay, HTLV(CR) RT confirmed cation choice for Mg(2+) over Mn(2+), distinct from the traits of cellular DNA polymerases purified from human lymphocytes and the RT from most sort C viruses.

Antibodies to cellular DNA polymerase gamma and anti-bodies in opposition to RT purified from a number of animal retroviruses did not detectably work together with HTLV(CR) RT beneath circumstances that have been constructive for the respective homologous DNA polymerase, demonstrating a scarcity of shut relationship of HTLV(CR) RT to cellular DNA polymerases gamma or RT of those viruses. Six main proteins, with sizes of roughly 10,000, 13,000, 19,000, 24,000, 42,000, and 52,000 daltons, have been obvious when doubly banded, disrupted HTLV(CR) particles have been chromatographed on a NaDodSO(4)/polyacrylamide gel. The variety of these particle-associated proteins is in line with the anticipated proteins of a retrovirus, however the sizes of some are distinct from these of most identified retroviruses of the primate subgroups.

U myszy z niedoborem interleukiny 10 rozwija się przewlekłe zapalenie jelit. 

Interleukin-10 (IL-10) impacts the expansion and differentiation of many hemopoietic cells in vitro; particularly, it’s a potent suppressor of macrophage and T cell features. In IL-10-deficient mice, generated by gene concentrating on, lymphocyte growth and antibody responses are regular, however most animals are development retarded and anemic and endure from persistent enterocolitis. Alterations in gut embody intensive mucosal hyperplasia, inflammatory reactions, and aberrant expression of main histocompatibility complicated class II molecules on epithelia.

In distinction, mutants stored beneath particular pathogen-free circumstances develop solely a neighborhood irritation restricted to the proximal colon. These outcomes point out that the bowel irritation within the mutants originates from uncontrolled immune responses stimulated by enteric antigens and that IL-10 is a necessary immunoregulator within the intestinal tract.

Prosta technika ilościowego określania niskich poziomów uszkodzeń DNA w poszczególnych komórkach. 

Human lymphocytes have been both uncovered to X-irradiation (25 to 200 rads) or handled with H2O2 (9.1 to 291 microM) at Four levels C and the extent of DNA migration was measured utilizing a single-cell microgel electrophoresis approach beneath alkaline circumstances. Each brokers induced a major enhance in DNA migration, starting on the lowest dose evaluated.

Migration patterns have been comparatively homogeneous amongst cells uncovered to X-rays however heterogeneous amongst cells handled with H2O2. An evaluation of restore kinetics following publicity to 200 rads X-rays was performed with lymphocytes obtained from three people. The majority of the DNA restore occurred throughout the first 15 min, whereas all the restore was primarily full by 120 min after publicity. Nevertheless, some cells demonstrated no restore throughout this incubation interval whereas different cells demonstrated DNA migration patterns indicative of extra injury than that induced by the preliminary irradiation with X-rays. This method seems to be delicate and helpful for detecting injury and restore in single cells.

Zaangażowanie immunoinhibitorowego receptora PD-1 przez nowego członka rodziny B7 prowadzi do negatywnej regulacji aktywacji limfocytów.

 

PD-1 is an immunoinhibitory receptor expressed by activated T cells, B cells, and myeloid cells. Mice poor in PD-1 exhibit a breakdown of peripheral tolerance and reveal a number of autoimmune options. We report right here that the ligand of PD-1 (PD-L1) is a member of the B7 gene household. Engagement of PD-1 by PD-L1 results in the inhibition of T cell receptor-mediated lymphocyte proliferation and cytokine secretion. As well as, PD-1 signaling can inhibit at the very least suboptimal ranges of CD28-mediated costimulation. PD-L1 is expressed by antigen-presenting cells, together with human peripheral blood monocytes stimulated with interferon gamma, and activated human and murine dendritic cells.

As well as, PD-L1 is expressed in nonlymphoid tissues similar to coronary heart and lung. The relative ranges of inhibitory PD-L1 and costimulatory B7-1/B7-2 indicators on antigen-presenting cells might decide the extent of T cell activation and consequently the brink between tolerance and autoimmunity. PD-L1 expression on nonlymphoid tissues and its potential interplay with PD-1 might subsequently decide the extent of immune responses at websites of irritation.

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