Izolacja retrowirusa limfotropowego T od pacjenta
- Janusz
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Izolacja retrowirusa limfotropowego T od pacjenta z ryzykiem zespołu nabytego niedoboru odporności (AIDS).
A retrovirus belonging to the household of just lately found human T-cell leukemia viruses (HTLV), however clearly distinct from every earlier isolate, has been remoted from a Caucasian affected person with indicators and signs that usually precede the acquired immune deficiency syndrome (AIDS). This virus is a typical type-C RNA tumor virus, buds from the cell membrane, prefers magnesium for reverse transcriptase exercise, and has an inside antigen (p25) just like HTLV p24.
Antibodies from serum of this affected person react with proteins from viruses of the HTLV-I subgroup, however type-specific antisera to HTLV-I don’t precipitate proteins of the brand new isolate. The virus from this affected person has been transmitted into wire blood lymphocytes, and the virus produced by these cells is just like the unique isolate. From these research it’s concluded that this virus in addition to the earlier HTLV isolates belong to a normal household of T-lymphotropic retroviruses which are horizontally transmitted in people and could also be concerned in a number of pathological syndromes, together with AIDS.
CXCL1 CRISPR Knock Out L3.6pl Cell Line |
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T6221 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
Ccl2 CRISPR Knock Out CT26 Cell Line |
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T9611 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
TRPV6 CRISPR Knock Out Human PC3-M Cell Line |
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T6219 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
FOLR CRISPR Knock Out Human OVCAR3-A1 Cell Line |
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T6229 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
MUC16 CRISPR Knock Out Human OVCAR3-A1 Cell Line |
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T6230 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
NR2F6 CRISPR Knock Out Human Jurkat E6.1 Cell Line - Clone T3-10 |
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T6822 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
Aldh2 CRISPR Knock Out L929 Cell Line - Clone T3-20 |
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T9692 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
TREM2 CRISPR Knock Out Human THP-1 Cell Line - Clone T1-9 |
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T6810 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
PTPN6 CRISPR Knock Out Human THP-1 Cell Line - Clone T3-1 |
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T6813 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
PTPN11 CRISPR Knock Out NIH/3T3 Cell Line - Clone T4-9 |
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T6803 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
METTL3 CRISPR Knock Out Caki-1 Cell Line - Clone T2-16 |
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T9623 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Nacc1 CRISPR Knock Out ID8 Cell Line (Mouse) - Clone T1-23 |
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T9627 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
osbpl2 CRISPR Knock Out NIH/3T3 Cell Line - T1 Polyclonal Pool |
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T9682 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
GLT1 CRISPR Knock Out Rat AST Cell Line - Clone T2-1 |
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T9678 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
PRSS21 CRISPR Knock Out Human OVCAR8 Cell Line - Clone T2 C8 |
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T6811 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
SEC61G CRISPR Knock Out HCC-LM3 Cell Line - Clone T2-17-5 |
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T9746 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
Aifm1 CRISPR Knock Out Mouse L929 Cell Line - Clone T2-15-3 |
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T9674 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
MSLN CRISPR Knock Out Human OVCAR3-A1 Cell Line - Clone T2 C4 |
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T6820 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
CARMIL1 CRISPR Knock Out Immortalized Human Gingival Fibroblast - hTERT Cell Line - T1-2 |
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T6825 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
hPIM1 CRISPR Knock Out SACC-83 Cell Line-T3-9 |
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T9787 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
Dcx CRISPR Knock Out C6 Cell Line (Rat) - Clone sg4-1 |
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T9624 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Cell line gene knock-in and knock-out |
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S301 | 101Bio | - | EUR 15000 |
Human LY96 knockout cell line |
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ABC-KH8810 | AcceGen | 1 vial | Ask for price |
Description: Human LY96 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY96 knockdown cell line |
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ABC-KD8810 | AcceGen | 1 vial | Ask for price |
Description: Human LY96 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
SIRT1 Knockout Stable 293T Cell Line |
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T3176 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T1-19 |
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T9511 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Araf CRISPR Knocked out RAW264.7 Stable cell Cell Line (Mouse) T3-3 |
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T9529 | ABM | 1x10^6 cells/1.0ml | EUR 3950 |
T47D/GFP Cell Line |
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AKR-208 | Cell Biolabs | 1 vial | EUR 686.4 |
Description: T47D/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
Stable Mouse Myeloid-derived Suppressor-like LAL Knock Out (HD1B) Cell Line |
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T3187 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
293T [HEK-293T] Cell Line |
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CL-0005 | Elabscience Biotech | 1×10⁶ cells/vial | EUR 420 |
Description: Homo sapiens, Human |
293T Cell Line |
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LV010 | ABM | 1x10^6 | EUR 450 |
293T/17 Cell Line |
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CL-0469 | Elabscience Biotech | 1×10⁶ cells/vial | EUR 500 |
Description: Homo sapiens, Human |
293T Cell Lysate (Human embryonic kidney cell line) |
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LF-R0006 | Abfrontier | 200 ul | EUR 103.2 |
Description: 293T (Human embryonic kidney cell line) Whole Cell Lysate |
293AD Cell Line |
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AD-100 | Cell Biolabs | 1 vial | EUR 553.2 |
Description: The 293AD Cell Line is derived from the parental 293 cells but selected for attributes that increase adenovirus production, including firmer attachment and larger surface area. |
293AAV Cell Line |
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AAV-100 | Cell Biolabs | 1 vial | EUR 609.6 |
Description: The 293AAV Cell Line is derived from the parental 293 cells but selected for attributes that increase AAV production, including firmer attachment and larger surface area. |
293LTV Cell Line |
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LTV-100 | Cell Biolabs | 1 vial | EUR 609.6 |
Description: The 293LTV Cell Line is derived from the parental 293 cells but selected for attributes that increase lentiviral production, including fimrer attachment and larger surface area. |
293RTV Cell Line |
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RV-100 | Cell Biolabs | 1 vial | EUR 609.6 |
Description: The 293RTV Cell Line is derived from the parental 293 cells but selected for attributes that increase retroviral production, including fimrer attachment and larger surface area. |
Cas9 Expressing 293T Cell Line |
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T3251 | ABM | 1x10^6 cells / 1.0 ml | EUR 795 |
293/GFP Cell Line |
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AKR-200 | Cell Biolabs | 1 vial | EUR 686.4 |
Description: 293/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
A549/GFP Cell Line |
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AKR-209 | Cell Biolabs | 1 vial | EUR 686.4 |
Description: A549/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
HeLa/GFP Cell Line |
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AKR-213 | Cell Biolabs | 1 vial | EUR 686.4 |
Description: HeLa/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
293/Cas9 Cell Line |
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AKR-5110 | Cell Biolabs | 1 vial | EUR 686.4 |
HeLa/Cas9 Cell Line |
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AKR-5111 | Cell Biolabs | 1 vial | EUR 686.4 |
NIH3T3/GFP Cell Line |
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AKR-214 | Cell Biolabs | 1 vial | EUR 686.4 |
Description: NIH3T3/GFP Cell Line stably expresses GFP and otherwise exhibits the same characteristics of the parental cell line. |
NIH3T3/Cas9 Cell Line |
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AKR-5104 | Cell Biolabs | 1 vial | EUR 686.4 |
Human LY75 knockout cell line |
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ABC-KH8806 | AcceGen | 1 vial | Ask for price |
Description: Human LY75 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY86 knockout cell line |
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ABC-KH8808 | AcceGen | 1 vial | Ask for price |
Description: Human LY86 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Blank Promoter Stable Expressing Human 293T Cell Line |
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T9618 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
MCF-7/Luc Cell Line |
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AKR-234 | Cell Biolabs | 1 vial | EUR 686.4 |
Description: MCF-7/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line. |
SKOV-3/Luc Cell Line |
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AKR-232 | Cell Biolabs | 1 vial | EUR 686.4 |
Description: SKOV-3/Luc Cell Line stably expresses luciferase and otherwise exhibits the same characteristics of the parental cell line. |
Stable 293T-ATD cell line |
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T6340 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
OVCAR-5/RFP Cell Line |
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AKR-254 | Cell Biolabs | 1 vial | EUR 686.4 |
Description: OVCAR-5/RFP Cell Line stably expresses RFP and otherwise exhibits the same characteristics of the parental cell line. |
Human LY75 knockdown cell line |
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ABC-KD8806 | AcceGen | 1 vial | Ask for price |
Description: Human LY75 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY86 knockdown cell line |
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ABC-KD8808 | AcceGen | 1 vial | Ask for price |
Description: Human LY86 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY75-CD302 knockout cell line |
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ABC-KH8807 | AcceGen | 1 vial | Ask for price |
Description: Human LY75-CD302 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
PTPN6 Stable Knocked Out THP-1 Cell Line |
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T6216 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
SIRT1 Wildtype Stable 293T Cell Line |
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T3175 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Mouse NACC1 CRISPR Stable Knockout ID8 Cell Line - Clone T1-23 |
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T6826 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Human LY9 knockout cell line |
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ABC-KH8809 | AcceGen | 1 vial | Ask for price |
Description: Human LY9 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY6D knockout cell line |
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ABC-KH8797 | AcceGen | 1 vial | Ask for price |
Description: Human LY6D knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY6E knockout cell line |
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ABC-KH8798 | AcceGen | 1 vial | Ask for price |
Description: Human LY6E knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY6H knockout cell line |
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ABC-KH8804 | AcceGen | 1 vial | Ask for price |
Description: Human LY6H knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY6K knockout cell line |
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ABC-KH8805 | AcceGen | 1 vial | Ask for price |
Description: Human LY6K knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY6G5B knockout cell line |
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ABC-KH8799 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G5B knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY6G5C knockout cell line |
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ABC-KH8800 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G5C knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY6G6C knockout cell line |
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ABC-KH8801 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G6C knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY6G6D knockout cell line |
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ABC-KH8802 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G6D knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY6G6F knockout cell line |
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ABC-KH8803 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G6F knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human LY75-CD302 knockdown cell line |
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ABC-KD8807 | AcceGen | 1 vial | Ask for price |
Description: Human LY75-CD302 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
PGC-1α/ERRα Stable 293T Cell Line |
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T3060 | ABM | 1x10^6 cells / 1.0 ml | EUR 385 |
Platinum-E Retroviral Packaging Cell Line, Ecotropic |
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RV-101 | Cell Biolabs | 1 vial | EUR 1104 |
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-E cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection. |
Platinum-GP Retroviral Packaging Cell Line, Pantropic |
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RV-103 | Cell Biolabs | 1 vial | EUR 1104 |
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-GP cells contain the gag and pol genes required for retroviral packaging; an expression vector is co-transfected with a VSVG envelope vector. |
Platinum-A Retroviral Packaging Cell Line, Amphotropic |
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RV-102 | Cell Biolabs | 1 vial | EUR 1104 |
Description: Conventional cells used for retrovirus packaging, such as those based on NIH3T3 cells, have limited stability and produce relatively low yields of retrovirus, mainly due to the poor expression of retroviral structure proteins (gag, pol and env) in the cells. The Platinum Retroviral Packaging Cell Lines are based on the 293T cell line. They exhibit longer stability and produce higher yields of retroviral structure proteins. Plat-A cells contain gag, pol and env genes, allowing retroviral packaging with a single plasmid transfection. |
Total Protein - Murine Embryonic Stem Cell Line D3 |
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CBA-305 | Cell Biolabs | 500 ?g | EUR 414 |
Description:
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Human LY9 knockdown cell line |
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ABC-KD8809 | AcceGen | 1 vial | Ask for price |
Description: Human LY9 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Mouse R-spondin1 Stable 293T Cell Line |
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T3156 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Human LY6D knockdown cell line |
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ABC-KD8797 | AcceGen | 1 vial | Ask for price |
Description: Human LY6D knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY6E knockdown cell line |
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ABC-KD8798 | AcceGen | 1 vial | Ask for price |
Description: Human LY6E knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY6H knockdown cell line |
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ABC-KD8804 | AcceGen | 1 vial | Ask for price |
Description: Human LY6H knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY6K knockdown cell line |
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ABC-KD8805 | AcceGen | 1 vial | Ask for price |
Description: Human LY6K knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY6G5B knockdown cell line |
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ABC-KD8799 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G5B knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY6G5C knockdown cell line |
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ABC-KD8800 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G5C knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY6G6C knockdown cell line |
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ABC-KD8801 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G6C knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY6G6D knockdown cell line |
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ABC-KD8802 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G6D knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LY6G6F knockdown cell line |
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ABC-KD8803 | AcceGen | 1 vial | Ask for price |
Description: Human LY6G6F knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
PFN2 Stable Expressing 293T Cell Line - Polyclonal |
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T9606 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
TARGATT? Knock-in Mouse Cell Line Generation Kit (Master Cell Line) |
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AST-7001 | Applied StemCell | 1 Kit | Ask for price |
Description: 6 month |
BRCA1 CRISPR Stable Knockout Human Fibroblast Cell Line |
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T6245 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Human 293T Cell Lysate Lyophilized |
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IHU293TTLLY200UG | Innovative research | each | EUR 89 |
Description: Human 293T Cell Lysate Lyophilized |
TARGATT? Knock-in iPSC Generation (Master Cell Line) |
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AST-1100 | Applied StemCell | 1 vial of 1X10^6 cells | Ask for price |
Description: 6 month |
YBX1 Stable Knockout Human NK/T Cell Line #T3-16 P6 |
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T9508 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
Ly96 3'UTR GFP Stable Cell Line |
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TU262699 | ABM | 1.0 ml | Ask for price |
Ly96 3'UTR GFP Stable Cell Line |
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TU162731 | ABM | 1.0 ml | Ask for price |
LY96 3'UTR GFP Stable Cell Line |
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TU062782 | ABM | 1.0 ml | EUR 1672.8 |
Human CRISP1 knockout cell line |
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ABC-KH3569 | AcceGen | 1 vial | Ask for price |
Description: Human CRISP1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human CRISP2 knockout cell line |
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ABC-KH3570 | AcceGen | 1 vial | Ask for price |
Description: Human CRISP2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human CRISP3 knockout cell line |
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ABC-KH3571 | AcceGen | 1 vial | Ask for price |
Description: Human CRISP3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
PTPRA CRISPR Knockout Immortalized Human Lung Fibroblast Cell Line |
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T6827 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
Human 293T Whole Cell Lysate |
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LYSATE0032 | BosterBio | 200ug | EUR 180 |
Description: This cell lysate is prepared from human 293T using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C. |
VEGF Promoter Stable Expressing 293T Cell Line - Clone #2 |
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T9622 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Human CRISPLD1 knockout cell line |
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ABC-KH3572 | AcceGen | 1 vial | Ask for price |
Description: Human CRISPLD1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human CRISPLD2 knockout cell line |
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ABC-KH3573 | AcceGen | 1 vial | Ask for price |
Description: Human CRISPLD2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Ly96 3'UTR Luciferase Stable Cell Line |
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TU112731 | ABM | 1.0 ml | Ask for price |
Ly96 3'UTR Luciferase Stable Cell Line |
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TU212699 | ABM | 1.0 ml | Ask for price |
LY96 3'UTR Luciferase Stable Cell Line |
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TU012782 | ABM | 1.0 ml | EUR 1672.8 |
AAVS1 Positive Control EGIP 293T Reporter Cell Line |
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CAS606A-1 | SBI | 1 Vial | EUR 726 |
Acan CRISPR Knockout RCS Cell Line (Rat) |
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T3000 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
TARGATT? Knock-in CHO Generation Kit (Master Cell Line) |
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AST-1200 | Applied StemCell | 1 Kit | Ask for price |
Description: 12 month |
Human CRISP1 knockdown cell line |
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ABC-KD3569 | AcceGen | 1 vial | Ask for price |
Description: Human CRISP1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human CRISP2 knockdown cell line |
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ABC-KD3570 | AcceGen | 1 vial | Ask for price |
Description: Human CRISP2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human CRISP3 knockdown cell line |
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ABC-KD3571 | AcceGen | 1 vial | Ask for price |
Description: Human CRISP3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
TARGATT? Knock-in HEK293 Generation Kit (Master Cell Line) |
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AST-1300 | Applied StemCell | 1 Kit | Ask for price |
Description: 12 month |
Human CRISPLD1 knockdown cell line |
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ABC-KD3572 | AcceGen | 1 vial | Ask for price |
Description: Human CRISPLD1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human CRISPLD2 knockdown cell line |
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ABC-KD3573 | AcceGen | 1 vial | Ask for price |
Description: Human CRISPLD2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
GFAT1 CRISPR Stable Knockout H1299 Cell Line |
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T6199 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
EDC4 CRISPR Stable Knockout HEK293T Cell Line |
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T6241 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
DJ-1 CRISPR Stable Knockout HeLa Cell Line |
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T6181 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
PD-L1 CRISPR Stable Knockout CT26 Cell Line |
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T6237 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
PDPN CRISPR Stable Knockout COS-7 Cell Line |
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T6039 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
PD-L1 CRISPR Stable Knockout MC38 Cell Line |
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T6149 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
LIMD1-/- CRISPR Stable Knockout HeLa Cell Line |
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T6357 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
LY96 sgRNA CRISPR Lentivector set (Human) |
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K1246301 | ABM | 3 x 1.0 ug | EUR 406.8 |
SLC35A1 CRISPR Stable Knockout CHO-S Cell Line |
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T6040 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
CXCL1 & CXCL8 CRISPR Double Knockout L3.6pl Cell Line |
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T6047 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
hCETP shRNA (Target C) Knock Down THP-1 Cell Line-Polyclone |
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T9789 | ABM | 1x10^6 cells / 1.0 ml | Ask for price |
AXL CRISPR Stable Knockout HT1080 (1-IV) Cell Line |
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T6232 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
AXL CRISPR Stable Knockout HT1080 (3-II) Cell Line |
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T6233 | ABM | 1x10^6 cells / 1.0 ml | EUR 3950 |
Human CRIM1 knockout cell line |
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ABC-KH3563 | AcceGen | 1 vial | Ask for price |
Description: Human CRIM1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human CRIP1 knockout cell line |
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ABC-KH3564 | AcceGen | 1 vial | Ask for price |
Description: Human CRIP1 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human CRIP2 knockout cell line |
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ABC-KH3565 | AcceGen | 1 vial | Ask for price |
Description: Human CRIP2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human CRIP3 knockout cell line |
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ABC-KH3566 | AcceGen | 1 vial | Ask for price |
Description: Human CRIP3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human CRIPT knockout cell line |
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ABC-KH3568 | AcceGen | 1 vial | Ask for price |
Description: Human CRIPT knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human CRIPAK knockout cell line |
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ABC-KH3567 | AcceGen | 1 vial | Ask for price |
Description: Human CRIPAK knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
LY96 sgRNA CRISPR Lentivector (Human) (Target 1) |
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K1246302 | ABM | 1.0 ug DNA | EUR 184.8 |
LY96 sgRNA CRISPR Lentivector (Human) (Target 2) |
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K1246303 | ABM | 1.0 ug DNA | EUR 184.8 |
LY96 sgRNA CRISPR Lentivector (Human) (Target 3) |
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K1246304 | ABM | 1.0 ug DNA | EUR 184.8 |
Cas9 (CRISPR Associated Protein 9) ELISA Kit |
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PRB-5079 | Cell Biolabs | 96 assays | EUR 686.4 |
Cas13a (CRISPR Associated Protein 13a, C2c2) ELISA Kit |
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PRB-5091 | Cell Biolabs | 96 assays | EUR 686.4 |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
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CBA-135 | Cell Biolabs | 96 assays | EUR 985.2 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Colorimetric |
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CBA-135-5 | Cell Biolabs | 5 x 96 assays | EUR 4027.2 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric |
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CBA-140 | Cell Biolabs | 96 assays | EUR 1027.2 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
CytoSelect Cell Transformation Assay (Cell Recovery Compatible), Fluorometric |
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CBA-140-5 | Cell Biolabs | 5 x 96 assays | EUR 4179.6 |
Description: CytoSelect 96-Well Cell Transformation Assays (Cell Recovery Compatible) provide a robust system for detecting transformed cells, screening cell transformation inhibitors, and determining in vitro drug sensitivity. A proprietary modified soft agar matrix allows you to either quantify cells using the included fluorescent dye, or recover the cells for further analysis. |
StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) |
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CBA-325 | Cell Biolabs | 96 assays | EUR 1027.2 |
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis. |
StemTAG Stem Cell Colony Formation Assay (Cell Recovery Compatible) |
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CBA-325-5 | Cell Biolabs | 5 x 96 assays | EUR 4033.2 |
Description: Our StemTAG 96-Well Stem Cell Colony Formation Assay provides a high-throughput method to quantify ES cells in just 7-10 days, and no manual cell counting is required. Once colonies are formed, they may be analyzed in three different ways: 1. Lyse cells, then quantify in a fluorescence plate reader using dye included in the kit; 2. Lyse cells, then quantify alkaline phosphatase activity using reagents provided; or 3. Recover colonies from matrix for further culture or analysis. |
Human CRIM1 knockdown cell line |
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ABC-KD3563 | AcceGen | 1 vial | Ask for price |
Description: Human CRIM1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human CRIP1 knockdown cell line |
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ABC-KD3564 | AcceGen | 1 vial | Ask for price |
Description: Human CRIP1 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human CRIP2 knockdown cell line |
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ABC-KD3565 | AcceGen | 1 vial | Ask for price |
Description: Human CRIP2 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human CRIP3 knockdown cell line |
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ABC-KD3566 | AcceGen | 1 vial | Ask for price |
Description: Human CRIP3 knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human CRIPT knockdown cell line |
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ABC-KD3568 | AcceGen | 1 vial | Ask for price |
Description: Human CRIPT knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human CRIPAK knockdown cell line |
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ABC-KD3567 | AcceGen | 1 vial | Ask for price |
Description: Human CRIPAK knockdown cell line is engineered by our optimized transduction of the specific shRNA with lentivirus. Knockdown levels are determined via qRT-PCR. Gentaur offers generation of stable knockdown (RNAi) cell lines expressing shRNAs targeting genes of your interest. |
Human LIN37 knockout cell line |
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ABC-KH8476 | AcceGen | 1 vial | Ask for price |
Description: Human LIN37 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human KY knockout cell line |
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ABC-KH8250 | AcceGen | 1 vial | Ask for price |
Description: Human KY knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human MB knockout cell line |
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ABC-KH9065 | AcceGen | 1 vial | Ask for price |
Description: Human MB knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human F2 knockout cell line |
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ABC-KH5009 | AcceGen | 1 vial | Ask for price |
Description: Human F2 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human F3 knockout cell line |
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ABC-KH5014 | AcceGen | 1 vial | Ask for price |
Description: Human F3 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human F5 knockout cell line |
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ABC-KH5015 | AcceGen | 1 vial | Ask for price |
Description: Human F5 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human F7 knockout cell line |
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ABC-KH5016 | AcceGen | 1 vial | Ask for price |
Description: Human F7 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human F8 knockout cell line |
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ABC-KH5017 | AcceGen | 1 vial | Ask for price |
Description: Human F8 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human F9 knockout cell line |
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ABC-KH5021 | AcceGen | 1 vial | Ask for price |
Description: Human F9 knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Human GC knockout cell line |
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ABC-KH5948 | AcceGen | 1 vial | Ask for price |
Description: Human GC knockout cell line is HEK293/HeLa cell line, edited by CRISPR/Cas9 technology. |
Wykrywanie i izolacja cząstek retrowirusa typu C ze świeżych i hodowanych limfocytów pacjenta ze skórnym chłoniakiem T-komórkowym.
Retrovirus particles with sort C morphology have been present in two T-cell lymphoblastoid cell strains, HUT 102 and CTCL-3, and in recent peripheral blood lymphocytes obtained from a affected person with a cutaneous T-cell lymphoma (mycosis fungoides). The cell strains constantly produce these viruses, that are collectively known as HTLV, pressure CR(HTLV(CR)). Initially, the manufacturing of virus from HUT 102 cells required induction with 5-iodo-2′-deoxyuridine, however the cell line grew to become a constitutive producer of virus at its 56th passage. Cell line CTCL-Three has been a constitutive producer of virus from its second passage in tradition.
Each mature and immature furthercellular virus particles have been seen in thin-section electron micrographs of fastened, pelleted cellular materials; once in a while, typical sort C budding virus particles have been seen. No type of intracellular virus particle has been seen. Mature particles have been 100-110 nm in diameter, consisted of an electron-dense core surrounded by an outer membrane separated by an electron-lucent area, banded at a density of 1.16 g/ml on a steady 25-65% sucrose gradient, and contained 70S RNA and a DNA polymerase exercise typical of viral reverse transcriptase (RT; RNA-dependent DNA nucleotidyltransferase). Underneath sure circumstances of assay, HTLV(CR) RT confirmed cation choice for Mg(2+) over Mn(2+), distinct from the traits of cellular DNA polymerases purified from human lymphocytes and the RT from most sort C viruses.
Antibodies to cellular DNA polymerase gamma and anti-bodies in opposition to RT purified from a number of animal retroviruses did not detectably work together with HTLV(CR) RT beneath circumstances that have been constructive for the respective homologous DNA polymerase, demonstrating a scarcity of shut relationship of HTLV(CR) RT to cellular DNA polymerases gamma or RT of those viruses. Six main proteins, with sizes of roughly 10,000, 13,000, 19,000, 24,000, 42,000, and 52,000 daltons, have been obvious when doubly banded, disrupted HTLV(CR) particles have been chromatographed on a NaDodSO(4)/polyacrylamide gel. The variety of these particle-associated proteins is in line with the anticipated proteins of a retrovirus, however the sizes of some are distinct from these of most identified retroviruses of the primate subgroups.
U myszy z niedoborem interleukiny 10 rozwija się przewlekłe zapalenie jelit.
Interleukin-10 (IL-10) impacts the expansion and differentiation of many hemopoietic cells in vitro; particularly, it’s a potent suppressor of macrophage and T cell features. In IL-10-deficient mice, generated by gene concentrating on, lymphocyte growth and antibody responses are regular, however most animals are development retarded and anemic and endure from persistent enterocolitis. Alterations in gut embody intensive mucosal hyperplasia, inflammatory reactions, and aberrant expression of main histocompatibility complicated class II molecules on epithelia.
In distinction, mutants stored beneath particular pathogen-free circumstances develop solely a neighborhood irritation restricted to the proximal colon. These outcomes point out that the bowel irritation within the mutants originates from uncontrolled immune responses stimulated by enteric antigens and that IL-10 is a necessary immunoregulator within the intestinal tract.
Prosta technika ilościowego określania niskich poziomów uszkodzeń DNA w poszczególnych komórkach.
Human lymphocytes have been both uncovered to X-irradiation (25 to 200 rads) or handled with H2O2 (9.1 to 291 microM) at Four levels C and the extent of DNA migration was measured utilizing a single-cell microgel electrophoresis approach beneath alkaline circumstances. Each brokers induced a major enhance in DNA migration, starting on the lowest dose evaluated.
Migration patterns have been comparatively homogeneous amongst cells uncovered to X-rays however heterogeneous amongst cells handled with H2O2. An evaluation of restore kinetics following publicity to 200 rads X-rays was performed with lymphocytes obtained from three people. The majority of the DNA restore occurred throughout the first 15 min, whereas all the restore was primarily full by 120 min after publicity. Nevertheless, some cells demonstrated no restore throughout this incubation interval whereas different cells demonstrated DNA migration patterns indicative of extra injury than that induced by the preliminary irradiation with X-rays. This method seems to be delicate and helpful for detecting injury and restore in single cells.
Zaangażowanie immunoinhibitorowego receptora PD-1 przez nowego członka rodziny B7 prowadzi do negatywnej regulacji aktywacji limfocytów.
PD-1 is an immunoinhibitory receptor expressed by activated T cells, B cells, and myeloid cells. Mice poor in PD-1 exhibit a breakdown of peripheral tolerance and reveal a number of autoimmune options. We report right here that the ligand of PD-1 (PD-L1) is a member of the B7 gene household. Engagement of PD-1 by PD-L1 results in the inhibition of T cell receptor-mediated lymphocyte proliferation and cytokine secretion. As well as, PD-1 signaling can inhibit at the very least suboptimal ranges of CD28-mediated costimulation. PD-L1 is expressed by antigen-presenting cells, together with human peripheral blood monocytes stimulated with interferon gamma, and activated human and murine dendritic cells.
As well as, PD-L1 is expressed in nonlymphoid tissues similar to coronary heart and lung. The relative ranges of inhibitory PD-L1 and costimulatory B7-1/B7-2 indicators on antigen-presenting cells might decide the extent of T cell activation and consequently the brink between tolerance and autoimmunity. PD-L1 expression on nonlymphoid tissues and its potential interplay with PD-1 might subsequently decide the extent of immune responses at websites of irritation.